Journal: Life Science Alliance

Article Title: Prostaglandin F2α regulates mitochondrial dynamics and mitophagy in the bovine corpus luteum

doi: 10.26508/lsa.202301968

Figure Lengend Snippet: Mid-cycle cows (n = 3/time-point) were administered I.M. PGF2α (25 mg) for 1, 2, and 4 h or control saline injections (n = 3). (A) Serum progesterone concentrations were obtained from animals 0, 1, 2, and 4 h following I.M PGF2α administration (n = 3 to 4 animals per time point). Statistics were performed by one-way ANOVA followed by Tukey's multiple comparison tests. (B) Luteal tissue progesterone concentrations (n = 5 saline-treated; n = 6 PGF2α-treated) 4 h post-I.M PGF2α treatment. Statistics were performed by t tests to evaluate paired responses. (C) Representative Western blot analysis of the phosphorylation of DRP1 and MFF in luteal tissue 1, 2, and 4 h after I.M administration of PGF2α. (D) Densitometric analyses of phospho-DRP1 (Ser616). (E) Densitometric analyses of phospho-DRP1 (Ser637). Symbols represent mean fold changes (means ± sem, n = 3). (F) Densitometric analyses of phospho-MFF (Ser146). Symbols represent mean fold changes (means ± sem, n = 3). Statistics performed by two-way ANOVA was used to evaluate repeated measures with Dunnett’s post tests to compare means. (G) Representative immunohistochemistry micrograph of the phosphorylation of DRP1 and MFF in luteal tissue 4 h after I.M administration of PGF2α treatment. Micron bar = 5 mm (10x) and 1 mm (40x). Significant difference between treatments compared with saline-treated animals, * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: An ELISA kit for progesterone was purchased from DRG International, Inc. ImmuChemTM Coated Tube Progesterone 125I RIA kit was purchased from ICN Pharmaceuticals, Inc. lists all antibodies used in the study.

Techniques: Control, Saline, Comparison, Western Blot, Phospho-proteomics, Immunohistochemistry